Defects of receptor-mediated low density lipoprotein catabolism in homozygous familial hypercholesterolemia and hypothyroidism in vivo.

Abstract
The role of low density lipoprotein (LDL) receptors, in the pathogenesis of hereditary and acquired forms of hypercholesterolemia, was investigated in vivo by simultaneously determining total and receptor-independent LDL catabolism with 125I-labeled LDL and 131I-labeled LDL coupled with cyclohexanedione. Receptor-mediated catabolism of LDL, determined as the difference between the turnover of 125I and 131I, was virtually absent in 2 homozygotes wih familial hypercholesterolemia and markedly reduced in a hypothyroid patient. Treatment of the latter with L-thyroxine markedly stimulated receptor-mediated catabolism and reduced LDL levels as did cholestyramine administration in a control subject. Reduction of LDL levels by plasma exchange in a control subject and homozygote had no such effect. An intrinsic and almost total defect of receptor-mediated LDL catabolism evidently exists in homozygous familial hypercholesterolemia, and an analogous but reversible abnormality in hypothyroidism is demonstrated.

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