Blastogenetic responsiveness of peripheral blood lymphocytes from patients with adult periodontitis, evaluated by a reverse hemolytic plaque assay

Publisher Website
Google Scholar
Abstract
Peripheral blood lymphocytes were obtained from 10 patients with advanced periodontitis and from 5 normal control subjects. The lymphocytes were cultured with dental plaque, supernatant of homologous fibroblast cytoplasm and Pokeweed-mitogen (PWM). The number of immunoglobulin-secreting cells (ISC) was assessed after 7 days using a reverse hemolytic plaque assay (RHPA). The stimulation levels of lymphocytes from periodonitis patients and control subjects stimulated with dental plaque and PWM were not statistically different (p > 0.05). In contrast, the number of ISC produced after stimulation with fibroblst cytoplasm was increased significantly in the patients group (p < 0.05). These data support the hypothesis that autologous substances play an important role in the cellular immune responses of patients with severe chronic inflammatory periodontal disease (CIPD).