Spectroscopy of Light Scattered by Suspended Charged Particles

Abstract
Two spectroscopic techniques of the Rayleigh scattered light have been investigated. One investigation is on a method to measure the Rayleigh linewidth precisely. From the measurements it is found that the linewidth of the scattered light by the polystyrene latex particles in the buffer solution is significantly different from that obtained from the Einstein-Stokes relation. The other investigation is on an application of the spectroscopic method for the detection of the electrophoretic analysis. From the investigation it is found that the Rayleigh scattered spectral line shows the Doppler shift by the electrophoretic movement of the particle and that the electrophoretic velocity can be measured in real time.

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