Pertussis toxin prevents presynaptic inhibition by kainate receptors of rat hippocampal [3H]GABA release
Open Access
- 8 March 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 469 (2-3) , 159-162
- https://doi.org/10.1016/s0014-5793(00)01272-2
Abstract
Kainate receptors are ionotropic receptors, also reported to couple to Gi/Go proteins, increasing neuronal excitability through disinhibition of neuronal circuits. We directly tested in hippocampal synaptosomes if kainate receptor‐mediated inhibition of GABA release involved a metabotropic action. The kainate analogue, domoate (3 μM), inhibited by 24% [3H]GABA‐evoked release, an effect reduced by 76% in synaptosomes pre‐treated with pertussis toxin. Protein kinase C inhibition attenuated by 82% domoate‐induced inhibition of GABA release whereas protein kinase C activation did not change kainate receptor binding. Thus, domoate inhibition of GABA release recruits Gi/Go proteins and a protein kinase C pathway.Keywords
This publication has 15 references indexed in Scilit:
- Limbic seizure and brain damage produced by kainic acid: Mechanisms and relevance to human temporal lobe epilepsyPublished by Elsevier ,2003
- Purinergic modulation of [3H]GABA release from rat hippocampal nerve terminalsNeuropharmacology, 2000
- Cross Talk Between A1 and A2A Adenosine Receptors in the Hippocampus and Cortex of Young Adult and Old RatsJournal of Neurophysiology, 1999
- Mechanisms underlying kainate receptor-mediated disinhibition in the hippocampusProceedings of the National Academy of Sciences, 1999
- Kainate Receptors Coupled to Gi/GoProteins in the Rat HippocampusMolecular Pharmacology, 1999
- On slices, synaptosomes and dissociated neurones to study in vitro ageing physiologyTrends in Neurosciences, 1998
- Inhibition of [3H]γ-aminobutyric acid release by kainate receptor activation in rat hippocampal synaptosomesEuropean Journal of Pharmacology, 1997
- Presynaptic inhibitory effect of baclofen on hippocampal inhibitory synaptic transmission involves a pertussis toxin-sensitive G-proteinEuropean Journal of Pharmacology, 1993
- Phosphorylation and modulation of recombinant GluR6 glutamate receptors by cAMP-dependent protein kinaseNature, 1993
- Adenosine receptors and calcium: Basis for proposing a third (A3) adenosine receptorProgress in Neurobiology, 1986