Mannheimia ( Pasteurella ) haemolytica Leukotoxin Binding Domain Lies within Amino Acids 1 to 291 of Bovine CD18

Abstract
Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia ( Pasteurella ) haemolytica . In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.