The Primary Structure of Valine-I Transfer Ribonucleic Acid from Torulopsis utilis

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Abstract
The primary structure of tRNA1Val from Torulopsis utilis has- been completely established by analyses of large oligonucleotides isolated from a partial digest of this RNA with RNase T1 [EC 2.7.7.26]. Methods for the partial degradation of the RNA, and the isolation and sequence determination of the oligonucleotides are described, together with the derivation of the total sequence for the RNA. The established sequence can be readily arranged in the “cloverleaf” model characteristic of Saccharomyces tRNA's of known structure. Although the primary structures of the tRNA's1Val from Torulopsis utilis and baker's yeast are closely similar, there are a few differences between them.

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