Effects of pressure on glucose transport in human erythrocytes

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Abstract
The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero‐trans and equilibrium exchange influx to similar extents, by decreasing the V max but not significantly changing the K m. The B max and K d of specific [3H]cytochalasin B binding were unaffected by pressure indicating no change to the number or affinity of functional transporters at pressure. Passive glucose transport was inhibited by pressure in a manner consistent with permeation across the lipid bilayer. These data indicate that there is a major change in volume during the translocation step of the glucose transporter which is rate‐limiting for transport.

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