Cell cycle and cell size dependence of susceptibility to hydrodynamic forces
- 5 April 1995
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 46 (1) , 88-92
- https://doi.org/10.1002/bit.260460112
Abstract
Exposure of animal cells to intense hydrodynamic forces exerted in turbulent capillary flow, and by controiled agitation and aeration, resulted in preferential destruction of S and G(2) cells and the extent of destruction of these cells was dependent upon the intensity of the action. The loss of these cells was possibly due to their larger size. However, the appearance of large numbers of membrane-bound vesicular structures similar to apoptotic bodies as well as cells with low DNA stainability (in a sub-G(1) peak) suggested that the action of adverse hydrodynamic forces on these large cells may at least in part be to induce an apoptotic response. (c) 1995 John Wiley & Sons, Inc.Keywords
This publication has 14 references indexed in Scilit:
- Cell death in bioreactors: A role for apoptosisBiotechnology & Bioengineering, 1994
- A flow cytometric study of hydrodynamic damage to mammalian cellsJournal of Biotechnology, 1993
- Multiple cell cycle access to the apoptotic death programme in human neuroblastoma cellsFEBS Letters, 1993
- Damaging agitation intensities increase DNA synthesis rate and alter cell‐cycle phase distributions of CHO cellsBiotechnology & Bioengineering, 1992
- A physical characterization of GAP A3 hybridoma cells: Morphology, geometry, and mechanical propertiesBiotechnology & Bioengineering, 1991
- Influence of serum level, cell line, flow type and viscosity on flow-induced lysis of suspended mammalian cellsBiotechnology Letters, 1989
- Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentrationJournal of Biotechnology, 1988
- Flow effects on the viability and lysis of suspended mammalian cellsBiotechnology Letters, 1987
- Lymphoma models for B cell activation and tolerance. III. Cell cycle dependence for negative signalling of WEHI-231 B lymphoma cells by anti-mu.The Journal of Experimental Medicine, 1986
- ORGANIZATION AND CELL CYCLE REGULATION OF HUMAN HISTONE GENESAnnals of the New York Academy of Sciences, 1982