Immunoprobes for polychlorinated dibenzodioxins: Synthesis of immunogen and characterization of antibodies

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Abstract
We have synthesized a novel dioxin immunogen, 2‐adipamide‐7, 8‐dichlorodibenzodioxin‐BSA, with a view to generating antibodies suitable for use in an immunoaffinity column. The specificity of antisera raised in rabbits to this conjugate was assessed in a competitive enzyme‐linked immunosorbent assay which was sensitive to 1 ng 2,3,7,8‐tetrachlorodibenzodioxin well‐1. Cross‐reactivity studies indicated that the antibodies were more sensitive to this congener than to the more highly chlorinated structures which are considered less toxic. 3,3’,4,4,‐Tetrachlorobiphenyl was unable—at any concentration—to displace antibody from the solid phase. We consider such specificity would be eminently suitable for immunoaffinity columns in the clean‐up and concentration of samples prior to dioxin determination by gas chromatography/mass spectrometry.