A combination of Coomassie Blue and concanavalin A ‐ based glycoprotein stains avoids ambiguities in the assignment of bands

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Abstract
A simple procedure is described to double‐stain sodium dodecyl sulfate‐polyacrylamide gels both with Coomassie Blue and by the concanavalin A ‐ horseradish peroxidase method to detect glycoproteins among the protein bands. Double staining proves useful in cases where many bands are present on the gels and where a comparison of banding patterns from separate gels or even separate lanes of the same gel cannot be carried out with sufficient accuracy.