Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I.
- 1 November 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (22) , 6908-6911
- https://doi.org/10.1073/pnas.79.22.6908
Abstract
The nucleotide sequence(s) specifying RNA polymerase I initiation has been investigated by studying the transcription of deleted and nondeleted mouse ribosomal RNA gene (rDNA) templates in vitro. The deletion of 5'-flanking sequences upstream from position -- 39 did not affect transcriptional activity, but removal of sequences between positions -- 39 and -- 34 resulted in a 90% decrease of rDNA transcription. The template activity was completely eliminated by the further deletion of nucleotides -- 33 to -- 13. It is concluded that sequences between -- 34 and -- 12, upstream from the transcribed region, represent an essential control region for the initiation of transcription in vitro. Therefore, this region may be functionally analogous to the T-A-T-A box of RNA polymerase II promoters. In addition to this control region, sequences located further upstream (between positions -- 45 and -- 169) may also exert some function in efficient transcription initiation as revealed by competition experiments between wildtype and mutant rDNA templates.This publication has 20 references indexed in Scilit:
- Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.Proceedings of the National Academy of Sciences, 1981
- Mapping of a mouse ribosomal DNA promoter by in vitro transcriptionNucleic Acids Research, 1981
- Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealisNucleic Acids Research, 1981
- Transcriptional Control Regions: Nucleotide Sequence Requirements for Initiation by RNA Polymerase II and IIIPublished by Springer Nature ,1981
- 5′ flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systemsCell, 1980
- The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transriptionNucleic Acids Research, 1980
- The nucleotide sequence of the initiation and termination sites for ribosomal rna transcription in x. laevisCell, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Control of RNA synthesis. 3. Control of ribonucleic acid synthesis in eukaryotes. 3. The effect of cycloheximide and edeine on RNA synthesis in yeastBiochemistry, 1976
- Amino acid starvation affects the initiation frequency of nucleolar RNA polymeraseCell, 1976