Acid Mucopolysaccharides in the Oral Epithelium

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Abstract
Methods of histochemical differentiation of acid mucopolysac-charides were applied to formalin fixed specimens of gingiva. Alcian-Blue (AB) (pH 2.5), Aldehyde -Fuchsin (AF) (pH 1.5), periodic acid Schiff AB (PAS), - PAS (pH 2.6), AF - AB, AB - AF Fuchsin were intercellulary; AB-PAS +Methylation (14 hrs. methanol in 0.1 N with HC1), AB-AF + Methylation, AF-AB +Methylation, Hematoxylin, Eosin-Y, Orange-G (Papanicoloau) were not found intercellulary ;Na2 35SO4 (inrat) was found only in the keratinizing layers. The intercellular mycopolysaccharides con-tainsulfatedacid mucopolysaccharides inadditionto neutral mucopoly-saccharides as indicated by staining at pH of 1.5 and by blockage of acid mucopoly saccharide staining by methylation. The Na2 35SO4 was in the highest concentration in the prekeratin and keratinizing areas of the epithelium. The Papanicoloau stain showed keratinization at the cell periphery in the keratinizing, but not the intercellulary layers. There is an increased concentration of sulfated mucopolysaccharides beginning in the prickle cell layer and ending in the keratinized layer. This is correlated to keratinlzation in sections stained with the Papanicoloau method and Na2 35SO4 autoradiograms. It is correlated to the increasing tonofibrlllae and desmosomes from lower to the keratinized layers of skin.

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