A Note on Poly-L-Lysine-Mediated Gene Transfer in HeLa Cells
Open Access
- 1 January 2003
- journal article
- research article
- Published by Taylor & Francis in Drug Delivery
- Vol. 10 (3) , 209-211
- https://doi.org/10.1080/713840395
Abstract
Poly-L-lysines of chain lengths varying from 70 to 300 residues are shown to bring about luciferase pRSVL DNA uptake and expression in HeLa cells. Transfection was approximately 50% that of the cationic liposome DOTAB. Expression was higher in the presence of chloroquine. Of interest was the fact that luciferase activity depended on the polysine/DNA charge ratio (+/−). Maximum activity occurred at a charge ratio (+/−) of 3, while at a charge ratio of 1 (conjugate electrically neutral) activity was much lower. At the higher charge ratios (+/−) of 4 and 5, luciferase activity decreased. The results obtained are discussed.Keywords
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