HPLC Separation of Cholecystokinin Peptides-Two Systems

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Abstract
Two HPLC [high performance liquid chromatography] systems are described which separate cholecystokinin (CCK) peptides from each other and from gastrin. One system utilizes a Supelcosil LC-18 reverse phase column with an acetonitrile-triethylamine phosphate buffer. The other system utilizes a Varian 2000 SW micropack molecular sieve column run in 0.1 M phosphate buffer. These HPLC systems offer improved resolution over conventional gel filtration of CCK and should be useful, when coupled with radioimmunoassay, for determining the molecular forms of CCK present in biological samples.