Isolation of malaria merozoites: release of Plasmodium chabaudi merozoites from schizonts bound to immobilized concanavalin A.

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Abstract
The ability of concanavalin A [Con-A] to bind erythrocytes but not malarial parasites was used for the development of a method of merozoite isolation: cells from infected blood were allowed to bind to a column of ConA linked to Sepharose beads and merozoites naturally released by maturation of the schizonts bound to the gel were collected. The principle of this method allowed its application to several Plasmodium spp. The kinetics of merozoite production and the quality of the preparations (purity, infectivity and ultrastructural morphology) were investigated by using P. chabaudi.