Identification of Etiological Leptospires of Weil's Disease Using Monoclonal Antibodies

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Abstract
Etiological agents of Weil's disease; Leptospira interrogans serovar icterohaemorrhagiae and serovar copenhageni, show remarkable cross-reactivity by the microscopic agglutination test against conventional immune animal sera. To facilitate reliable and rapid identification of the leptospires, we math 20 monoclonal antibodies by cell fusion technology. Hybridomas were produced through the fusion o: P3X63Ag8.653 cells with splenocytes obtained from BALB/c mice immunized against serovar icterohaemorrhagiae RGA strain and serovar copenhageni M 20 and Shiromizu strains. Reactivity of the antibodies produced by the hybridomas was determined by the agglutination test. By using 3 antibodie: of these hybridomas; SHIRMA 4 react to both serovars icterohaemorrhagiae and copenhageni leptospires RGAMA 1 react specifically to serovar icterohaemorrhagiae leptospires and SHIRMA 1 react specificall3 to serovar copenhageni leptospires, the 25 leptospiral strains were clearly classified by the agglutinatior test. All except 1 of 25 strains reacted to SHIRMA 4 and they also reacted to either of RGAMA 1 and SHIRMA 1. The 18 strains were identified as serovar icterohaemorrhagiae and the other 6 strains were identifiec as serovar copenhageni. Moreover, serovar copenhageni has been classified as the complete type anc serovar icterohaemorrhagiae has been classified as the incomplete type, but it has been definitely showr by using the monoclonal antibodies that each serovar has its own specific antigen (s) and the commor antigens. One strain which did not react to the 3 antibodies was identified as serovar autumnalis by the agglutination test against the immune rabbit sera.

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