A method for the determination of β-glucosidase activity in soil
Open Access
- 1 June 1973
- journal article
- research article
- Published by Taylor & Francis in Soil Science and Plant Nutrition
- Vol. 19 (2) , 103-108
- https://doi.org/10.1080/00380768.1973.10432524
Abstract
A method is described for the rapid and simple assay of soil β-glucosidase activity. It involves colorimetric estimation of ρ-nitrophenol released by β-glucosidase activity when soil is incubated in McIlvaine buffer (pH 4.8) with ρnitrophenyl βd-glucoside and toluene at 30°C for 1 hr. The method has been applied to three different soils. The range of β-glucosidase activity in cultivated soils was from 10.1 to 15.2 mµ mole per min per gram of dried soil. Km value for ρ-nitrophenyl β-d-glucoside was 3.3 × 10-4 M. Optimum pH was 4.8.Keywords
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