Investigation of Ultra-Low Temperature for Fungal Cultures. I. An Evaluation of Liquid-Nitrogen Storage for Preservation of Selected Fungal Cultures
- 1 May 1968
- journal article
- research article
- Published by Taylor & Francis in Mycologia
- Vol. 60 (3) , 613-621
- https://doi.org/10.1080/00275514.1968.12018610
Abstract
Mycelial culture-plugs of fungi, strains of Basidiomycetes predominating (74 of 104 strains studied), were successfully frozen with 10% (v/v) glycerol as suspending medium by a controlled slow freezing process. Frozen ampules were stored in a liquid-nitrogen refrigerator (—150 to —196 C). Eighteen-months' storage specimens became active, normal cultures when cultivated immediately succeeding the thawing (38 C).Keywords
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