Crystallization and preliminary X-ray crystallographic analysis of squalene-hopene cyclase from Alicyclobacillus acidocaldarius

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Abstract
The membrane‐associated protein squalene‐hopene cyclase from Alicyclobacillus acidocaldarius was overexpressed in Escherichia coli and purified by ion exchange and gel permeation chromatography. Crystals of three interrelated forms were grown by vapor diffusion under identical conditions. The crystals diffract to about 2.3 A resolution, but they are unstable in the X‐ray beam. An interpretable heavy‐atom derivative was obtained.
Funding Information
  • Deutsche Forschungsgemeinschaft (SFB-388)