Purification and immunochemical analysis of histidine decarboxylase from murine mastocytoma
- 1 April 1980
- journal article
- Published by Springer Nature in Inflammation Research
- Vol. 10 (1-2) , 92-93
- https://doi.org/10.1007/bf02024184
Abstract
Application of a new scheme of purification for histidine decarboxylase (E.C. 4.1.1.22) leads to a highly purified enzyme (5000 nmol/mg·h, i.p. 5.0, M.W.: 110 kD) with reasonable stability (rest activity 80% after 3 weeks at 6–8°C). A major protein contaminant seen on electrofocusing (i.p. 4.6) shows immunological identity with the enzyme-containing protein (i.p. 5.0) and might be involved in the aggregation of the enzyme.Keywords
This publication has 1 reference indexed in Scilit:
- Mammalian histidine decarboxylase; Changes in molecular properties induced by oxidation and reductionInflammation Research, 1980