The phosphorylation and absorption of sugars in the rat. 1. Hexokinase activity in the intestinal mucosa

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Abstract
The hexokinase reaction, the dephosphorylation of hexose phosphates, and adenosine triphosphatase activity were studied in dispersions of intestinal mucosa. Different sugars are phosphorylated at different rates, and hexokinase shows different patterns in response of the phosphorylation rates to changes in sugar concn. When the sugar concn. is not the limiting factor the rate with all the sugars examined varies in the same manner with the adenosine triphosphate concn. Sugars in mixtures show a depression in phosphorylation rate below that of single sugar controls. The difference in the observed phosphorylation rates of glucose and fructose is due to a property of the hexokinase, not to a difference in the rate of liberation of free sugar from the phosphorylated sugars formed, nor to the conditions of assay.