A Simple Procedure for the Isolation of Highly Purified Lysosomes from Normal Rat Liver

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Abstract
A procedure for the isolation of highly purified lysosomes from normal rat liver is described. The method depends on the swelling of mitochondria when the post-nuclear supernatant fraction is incubated with 1 him Ca2+. The lysosomes can then be separated from the swollen mitochondria by Percoll density gradient centrifu-gation. The lysosomal fraction obtained by our method was enriched more than 120-fold in terms of the marker enzymes with a yield of 25%. The electron microscopic examination and the measurement of the activities of marker enzymes for various subcellular organelles indicated that our lysosomal preparation was essentially free from contamination by other organelles.