Gangliosides of Human Chronic Lymphocytic Leukemia and Hairy Cells

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Abstract
Gangliosides were purified from the cells of 2 patients with hairy cell leukemia and 1 patient with chronic lymphocytic leukemia. Quantification of these compounds showed that these cells contain only 5-15% of the amount of lipid-bound sialic acid (gangliosides) per cell as normal lymphocytes. Structural characterization by gas-liquid chromatography, glycosidase treatment and high-performance liquid chromatography demonstrated that the major gangliosides of these leukemia cells were of the lactosyl type. Hairy cells contained monosialyllactosylceramide (II3NeuAc-LacCer); chronic lymphocytic leukemia cells contained monosialyllactosylceramide and disialyllactosylceramide (II3[NeuAc]2-LacCer). Chronic lymphocytic leukemia cells contained lesser amounts of 3 other gangliosides of the neolacto or lacto series as determined by endo-.beta.-galactosidase treatment. None of these leukemia cells contained detectable quantities of NeuAc-LcOse3Cer, a ganglioside found in normal leukocytes.

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