Separation of sulfated bile acids by high‐performance liquid chromatography
- 1 December 1978
- Vol. 13 (12) , 908-909
- https://doi.org/10.1007/bf02533848
Abstract
In order to establish a new method for simultaneous determination of sulfated bile acids without deconjugation, separation of 3-sulfates of unconjugated, glyco- and tauroconjugated bile acids by high-performance liquid chromatography has been undertaken. The preliminary experiment indicated that reversed phase chromatography on an ODS SC-02 column using ammonium carbonate aqueous solution/acetonitrile as a mobile phase would be promising. The ratio of peak width to retention time was varied depending upon the salt concentration. The content of water in mobile phase exerted an influence on the capacity ratio to a certain extent, but not on the resolution. The use of 0.5% ammonium carbonate/acetonitrile (26∶8 and 20∶8, v/v) was found to be suitable for complete resolution of sulfated cholate, chenodeoxycholate, deoxycholate, lithocholate and their glyco- and tauro-conjugates.This publication has 7 references indexed in Scilit:
- Analysis of metabolic profiles of bile acids in urine using a lipophilic anion exchanger and computerized gas-liquid chromatorgaphy-mass spectrometryJournal of Lipid Research, 1977
- Synthesis of the specific monosulfates of cholic acidSteroids, 1975
- Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugatesJournal of Lipid Research, 1975
- Sulfated and Nonsulfated Bile Acids in Urine, Serum, and Bile of Patients with Hepatobiliary DiseasesGastroenterology, 1975
- Bile acid sulfates. I. Synthesis of lithocholic acid sulfates and their identification in human bileJournal of Lipid Research, 1971
- The formation of bile acid sulfates: a new pathway of bile acid metabolism in humans.Proceedings of the National Academy of Sciences, 1967