Immunoaffinity Column Cleanup/Liquid Chromatographic Determination of Aflatoxins: An Interlaboratory Study

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Abstract
Nine laboratories from Denmark, Finland, Norway, and Sweden participated in a collaborative study to evaluate an immunoaffinity column cleanup/liquid chromatographic determination of aflatoxins B and G. Samples of peanut, fig, maize gluten, soya expel-ler, and copra spiked with total aflatoxin concentrations ranging from 1.37 to 28.60 ng/g were distributed. Altogether, 13 samples were analyzed. Of these, 6 were pairs of blind duplicates, 1 was an unknown negative sample containing ¼0.1 ng/g, and 2 were practice samples. A statistical presentation of the results is given. Although the results obtained were individually corrected for recovery, they were lower than expected in most cases. In some cases, the recoveries were unacceptably low, particularly for aflatoxin G2. Relative standard deviations for method repeatability (RSDr) and reproducibility (RSDR) were good but generally better for the peanut and fig samples than for the others. Results were better for aflatoxins B1 and G1 than for aflatoxins B2 and G2. RSDR values varied from 9 to 35%, and RSDR values varied from 17 to 81%. Horwitz ratio values were >2.00 for 1 RSDR value and R values.

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