Production and Storage of Inoculum ofCercospora kikuchiifor Field Studies
- 1 January 1985
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 75 (2) , 183-185
- https://doi.org/10.1094/phyto-75-183
Abstract
A procedure is described for granulating mycelial inoculum of C. kikuchii [Cercospora leaf blight and purple seed stain of soybean] by dropwise addition of homogenized mixtures of sodium alginate, kaolin clay, and mycelium into a 0.25 M CaCl2 solution. After 6 mo. under refrigeration, the granular preparations produced profuse viable, infective conidia when air-dried granules were rehydrated and exposed to irradiation from sunlamps (10 min/12 h) at 25.degree. C. An average of 3.8 .times. 106 conidia were produced per gram of air-dried granules of 4 isolates of C. kikuchii. This technique should prove useful for storage and production of large quantities of inoculum when needed for field evaluation of disease resistance and fungicide evaluation.This publication has 6 references indexed in Scilit:
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