In Vitro Enhancement of AA-Degrading Activity in Human Plasma with the Plasminogen Activator Streptokinase

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Abstract
Radiolabeled [amyloid] protein AA was coupled to CNBr-activated Sepharose 6 MB and used as a substrate to determine the AA-degrading activity of enzymes in solution. The applicability of the substrate was tested with an elastase preparation known to have AA-degrading activity. The substrate was used to determine the AA-degrading activity in fractions of normal human serum in the presence and absence of the plasminogen activator streptokinase. The AA-degrading activity was increased in fractions in which streptokinase-induced plasminogen activation had occurred. The increase in activity was inhibited with antibodies to plasminogen. AA-degrading activity was increased in whole human plasma by the addition of streptokinase. [Protein AA is considered to be an acute-phase reactant which increased several-fold during inflammation.].