Continuous Ethanol Production from Concentrated Wood Hydrolysates in an Internal Membrane-Filtration Bioreactor

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Abstract
Continuous culture for the production of ethanol from wood hydrolysate was carried out in an internal membrane‐filtration bioreactor. The hydrolysate medium was sterilized at a relatively low temperature of 60 °C with the intention of reducing the formation of inhibitory compounds during the sterilization. The maximum ethanol concentration and productivity obtained in this study were 76.9 g/L and 16.9 g/L‐h, respectively, which were much higher than those (57.2–67 g/L and 0.3–1.0 g/L‐h) obtained in batch cultures using hydrolysate media sterilized at 60 °C. The productivity was also found to be much higher than that (6.7 g/L‐h) obtained in a continuous cell retention culture using a wood hydrolysate sterilized at 121 °C. These results show that the internal membrane‐filtration bioreactor in combination with low‐temperature sterilization could be very effective for ethanol production from wood hydrolysate.

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