Odorant-binding protein: localization to nasal glands and secretions.
- 1 July 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (13) , 4942-4946
- https://doi.org/10.1073/pnas.83.13.4942
Abstract
An odorant-binding protein (OBP) was isolated from bovine olfactory and respiratory mucosa. We have produced polyclonal antisera to this protein and report its immunohistochemical localization to mucus-secreting glands of the olfactory and respiratory mucosa. Although OBP was originally isolated as a pyrazine binding protein, both rat and bovine OBP also bind the odorants [3H]methyldihydrojasmonate and 3,7-dimethyl-octan-1-ol as well as 2-isobutyl-3-[3H]-methoxypyrazine. We detect substantial odorant-binding activity attributable to OBP in secreted rat nasal mucus and tears but not in saliva, suggesting a role for OBP in transporting or concentrating odorants.This publication has 28 references indexed in Scilit:
- Evidence for non-receptor odor discrimination using neuroblastoma cells as a model for olfactory cellsBrain Research, 1985
- Application of the avidin-biotin-peroxidase complex (ABC) method to the light microscopic localization of pituitary hormones.Journal of Histochemistry & Cytochemistry, 1982
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Existence of a common precursor to ACTH and endorphin in the anterior and intermediate lobes of the rat pituaitaryJournal of Supramolecular Structure, 1978
- Studies on the receptors to 5α-androst-16-en-3-one and 5α-androst-16-en-3α-ol in sow nasal mucosaBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Evidence for a Chromatographic Model of OlfactionThe Journal of general physiology, 1970
- Topography of the nasal glands in rats and some other mammalsThe Anatomical Record, 1964