Endogenous Angiotensin and Pressure Modulate Brain Angiotensinogen and AT 1A mRNA Expression

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Abstract
In the coarctation hypertension model, we showed both dissociation of plasma renin activity from cardiovascular-induced effects and the reversal of hypertension-induced responses by losartan. In this study, we investigated the effects of hypertension on the expression of brain renin-angiotensin system components and the simultaneous functional responses and effects of long-term angiotensin II (AT) receptor blockade on these responses. Rats were given vehicle or losartan for 9 days and subjected to subdiaphragmatic aortic constriction or sham surgery after 4 days of treatment. On the fifth postsurgical day, pressure and heart rate were measured in the conscious state; the brain was perfused and removed afterward. Sequential slices of brainstem were hybridized with 35 S-oligodeoxynucleotide probes for angiotensinogen, AT 1A , and AT 1B receptors and processed for autoradiography and densitometry. In vehicle-treated rats, hypertension was accompanied by tachycardia and marked increments in angiotensinogen and AT 1A mRNA expression in the cardiovascular system–controlling brainstem areas. In the nucleus tractus solitarii, AT 1A density was correlated with both pressure and heart rate values ( P P 1A mRNA expression. Hypertension and losartan did not change AT 1B mRNA expression. The hypertension-induced positive feedback on angiotensinogen and AT 1A mRNA expression supports the concept of a permissive role for brain angiotensin II in orchestrating circulatory responses during the development of hypertension. These data also explain the efficacy of long-term AT 1 receptor blockade to reverse hypertension-induced effects.