The localization of tRNA 2 Asp genes from Drosophila melanogaster by “in situ” hybridization

Abstract
Transfer RNA Asp was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated “in vitro” with Na [125I] and hybridized “in situ” to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNA 2 Asp to the left arm of the second chromosome in the regions 29 D and E.