Influence of Nutrient Limitation and Growth Rate on the Outer Membrane Proteins of Klebsiella aerogenes NCTC 418

Publisher Website
Google Scholar
Abstract
Four major proteins with MW of 78,000, 37,000, 34,000 and 20,000 were present in the envelope of K. aerogenes when cultured at a high specific growth rate. At lower growth rates, the protein content and composition of the envelope depended on the imposed nutrient limitation. Under K-, C-, S- and P-limited conditions, derepression of synthesis of limitation-specific proteins was observed, their apparent MW being 90,000, 48,000, 41,000 and 36,000, respectively. N-limited cells had no additional proteins. For a particular limiting nutrient, expression of the limitation-specific proteins was independent of the chemical or physical form in which the nutrient was supplied. Under K or S limitation the specific proteins were present maximally at the lowest imposed growth rate; under C limitation a maximum expression of these proteins was found at moderate growth rates. Evidently, limitation-specific proteins which are associated with the outer membrane function in the uptake of limiting nutrients or, possibly, limitation-releasing compounds.

This publication has 17 references indexed in Scilit: