Displacement chromatography on synthetic ion-exchange resins. 7. Separations using a strongly basic resin

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Abstract
The use of a strongly basic anion-exchange resin (Dowex 2) for the separation of amino acids by displacement chromatography was investigated. Mixtures of the following pairs of amino acids were separated: glycine-alanine, valine-proline, methionine-leucine, A mixture of serine and threonine did not separate. The isolation of glycine and L-alanine from a protein hydrolysate is described. The order of displacement of 14 amino acids was determined by displacing the mixture with dilute HC1 from a column of Dowex 2. This order was substantially the same as the order of pK2(NH+3) for the monocarboxylic amino acids, and the result is discussed in relation to the theory of Davies (1949). The use of a strongly basic resin in a simple scheme for the isolation of amino acids from complex mixtures is discussed.