Alloantibody‐induced platelet serotonin release is blocked by antibody to the platelet PL antigen
- 1 October 1986
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 64 (2) , 331-338
- https://doi.org/10.1111/j.1365-2141.1986.tb04126.x
Abstract
Anti-platelet antibodies are known to activate the platelet release mechanism, usually measured by [14C]serotonin secretion. We have made immunoglobulin preparations of antibodies to HLA antigens, blood group A and platelet-specific PlA1 antigen on the platelet surface and have defined the optimal conditions for [14C]serotonin secretion by washed platelets. For optimal [14C]serotonin release, platelets were washed in apyrase and suspended in Tyrode-albumin with Ca++ and Mg++. and fibrinogen. Anti-PlA1 at high concentration did not cause [14C]serotonin release and inhibited serotonin release by other alloantibodies to antigens on the platelet surface. This was shown to be a result of the inhibition of fibrinogen binding to the platelets by bound anti-PlA1.This publication has 12 references indexed in Scilit:
- Effect of the Binding of Anti-ZwaAntibodies on Platelet FunctionVox Sanguinis, 1984
- Reactions of immunoglobulin G-binding ligands with platelets and platelet-associated immunoglobulin G.Journal of Clinical Investigation, 1984
- Generation of three different fragments of bound C3 with purified factor I or serum. II. Location of binding sites in the C3 Fragments for Factors B and H, complement receptors , and bovine conglutininThe Journal of Experimental Medicine, 1983
- A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa.Journal of Clinical Investigation, 1983
- Isolation and immunologic characterization of the human platelet alloantigen, P1A1Molecular Immunology, 1979
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Specific Interaction of HLA Antibodies (Eluates) with Washed PlateletsBritish Journal of Haematology, 1977
- Detection of platelet isoantibodies by (3H)serotonin platelet release and its clinical application to the problem of platelet matching.Journal of Clinical Investigation, 1975
- The Use of Platelet Serotonin Release as a Sensitive Method for Detecting Anti‐Platelet Antibodies and a Plasma Anti‐Platelet Factor in Patients with Idiopathic Thrombocytopenic Purpura*British Journal of Haematology, 1973
- Aggregation of Blood Platelets by Adenosine Diphosphate and its ReversalNature, 1962