The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients
- 1 August 1987
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 95 (1) , 25-30
- https://doi.org/10.1017/s0031182000057516
Abstract
SUMMARY: Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0·45% become mature ookinetes. A single-step gradient centrifugation method on 12·5% Nycodenz® enriches both gametocytes and ookinetes.This publication has 12 references indexed in Scilit:
- Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte culturesMolecular and Biochemical Parasitology, 1986
- Sequential expression of antigens on sexual stages of Plasmodium falciparum accessible to transmission-blocking antibodies in the mosquito.The Journal of Experimental Medicine, 1985
- The development of Plasmodium ookinetes in vitro: an ultrastructural study including a description of meiotic divisionParasitology, 1985
- In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytesParasitology, 1985
- Biosynthesis of the target antigens of antibodies blocking transmission of Plasmodium falciparumMolecular and Biochemical Parasitology, 1984
- New Observations on Gametogenesis, Fertilization, and Zygote Transformation in Plasmodium gallinaceum1The Journal of Protozoology, 1984
- The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis by colloidal silica (Percoll) gradient centrifugationTransactions of the Royal Society of Tropical Medicine and Hygiene, 1982
- Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static culturesTransactions of the Royal Society of Tropical Medicine and Hygiene, 1982
- Plasmodium gallinaceum: Transmission-blocking immunity in chickensExperimental Parasitology, 1979
- Evidence for cyclic development and short-lived maturity in the gametocytes of Plasmodium falciparumTransactions of the Royal Society of Tropical Medicine and Hygiene, 1971