Association of morphological and RFLP markers in rice (Oryza sativaL.)
- 1 June 1995
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 38 (3) , 566-574
- https://doi.org/10.1139/g95-073
Abstract
Seventeen morphological marker genes were associated with restriction fragment length polymorphism markers in rice by using four F2 populations, each segregating for a few observable traits, and 14 near isogenic lines (NILs), each containing one morphological mutant gene. The location of five genes was confirmed on the basis of F2 analysis: Purple hull (Pr) (16.8 ± 7.9 cM away from RG163 on chromosome 4); Phenol staining (Ph) (20.8 ± 8.4 cM away from RG163 on chromosome 4); glabrous leaf and hull (gl-1) (14.3 ± 7.4 cM away from RG182, and 20.9 ± 8.3 cM from RG403 on chromosome 5); Brown pericarp (Rc) (12.5 ± 7.2 cM away from RG30 on chromosome 7); and lazy growth habit (la) (28.8 ± 9.4 cM away from RG118 on chromosome 11). In addition, 12 other morphological markers, including the agronomically important genes semi-dwarf (sd-1) and Pollen restoring factor (Rf-1) were tentatively associated with mapped DNA clones based on screening pairs of NILs.Key words: linkage mapping, morphological marker, rice, Oryza sativa L., restriction fragment length polymorphism.Keywords
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