USE OF DEAE-CELLULOSE FILTERS IN THE S1 NUCLEASE METHOD FOR BACTERIAL DEOXYRIBONUCLEIC-ACID HYBRIDIZATION
- 1 January 1980
- journal article
- research article
- Vol. A131 (2) , 151-155
Abstract
Polynucleotide sequence relatedness can be studied with the S1 nuclease method by a fast and accurate procedure using DEAE-cellulose filters (DE81 Whatman). After S1 treatment of DNA-DNA hybrid molecules formed in 0.42 M saline solution, free nucleotides (digestion products) but not DNA are eluted from DE81 filters in appropriate salt concentration such as 5% Na2HPO4 .cntdot. 12 H2O. The relative binding ratio and the thermal stability of heteroduplexes are determined for 7 strains of Enterobacteriaceae. The conclusions resulting from S1 nuclease and DE81 filters assay are similar to those obtained with S1 nuclease and trichloracetic acid precipitation.This publication has 4 references indexed in Scilit:
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