Low temperature induced non-culturability and killing ofVibrio vulnificus

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Abstract
Vibrio vulnificus cells progressively lose culturability during incubation at 5°C. This process is accelerated by the addition of supernatants from non-culturable cells obtained by incubation at 5°C for 17 days. Thus the organism apparently produces a factor upon cold incubation which is triggering or causing the decline in culturability. Reversing the temperature shift can restore a culturable population comparable in numbers to the original population, but this process is largely due to regrowth. A few cells retaining the ability to grow apparently utilize the substrates released by the moribund cells, thus mimicking resuscitation of the whole population.