Estimation of Somatic Cells in Milk by Filter-Deoxyribonucleic Acid Method with Indole
Open Access
- 1 August 1973
- journal article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 56 (8) , 1097-1101
- https://doi.org/10.3168/jds.s0022-0302(73)85312-3
Abstract
The filter-deoxyribonucleic acid method of estimating somatic cells in milk was modified by indole instead of diphenyla- mine for development of color. This re- duced the time required for development of color from 16 h to 10 min. The corre- lation coefficient with direct microscopic somatic cell count was .99 with fresh milk. Two-deoxy-D-ribose yielded a linear relationship between concentration and optical density and was useful as a standard. Effectiveness of preservation of milk for the filter-deoxyribonucleic acid method by sodium dichromate and freez- ing was established.Keywords
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