Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo
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- 2 December 2002
- journal article
- research article
- Published by Springer Nature in Nature Neuroscience
- Vol. 6 (1) , 21-27
- https://doi.org/10.1038/nn983
Abstract
Neural stem cells exist in the developing and adult nervous systems of all mammals, but the basic mechanisms that control their behavior are not yet well understood. Here, we investigated the role of Sonic hedgehog (Shh), a factor vital for neural development, in regulating adult hippocampal neural stem cells. We found high expression of the Shh receptor Patched in both the adult rat hippocampus and neural progenitor cells isolated from this region. In addition, Shh elicited a strong, dose-dependent proliferative response in progenitors in vitro. Furthermore, adeno-associated viral vector delivery of shh cDNA to the hippocampus elicited a 3.3-fold increase in cell proliferation. Finally, the pharmacological inhibitor of Shh signaling cyclopamine reduced hippocampal neural progenitor proliferation in vivo. This work identifies Shh as a regulator of adult hippocampal neural stem cells.Keywords
This publication has 51 references indexed in Scilit:
- Medulloblastoma Growth Inhibition by Hedgehog Pathway BlockadeScience, 2002
- Functional neurogenesis in the adult hippocampusNature, 2002
- Neuronal specification in the spinal cord: inductive signals and transcriptional codesNature Reviews Genetics, 2000
- More hippocampal neurons in adult mice living in an enriched environmentNature, 1997
- Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene functionNature, 1996
- Is there a neural stem cell in the mammalian forebrain?Trends in Neurosciences, 1996
- Generation of Neurons and Astrocytes from Isolated Cells of the Adult Mammalian Central Nervous SystemScience, 1992
- An analysis of the origins of the cholinergic and noncholinergic septal projections to the hippocampal formation of the ratJournal of Comparative Neurology, 1985
- Neurogenesis in the Adult Rat: Electron Microscopic Analysis of Light RadioautographsScience, 1977
- Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in ratsJournal of Comparative Neurology, 1965