High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells
- 30 November 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 215 (3) , 491-503
- https://doi.org/10.1042/bj2150491
Abstract
The carriers of the carbohydrate differentiation antigens I, i and SSEA-1 were investigated in embryonal carcinoma cell lines of mouse [F9, PSA-4, MCP-6BRA] and differentiated cell lines derived from the [PSA-5E, RF9, MP2]. Glycoproteins were studied by immunostaining (Western blotting) of total cell lysates and immunoprecipitation from lysates of galactose oxidase/NaB3H4-labeled cells; glycolipids were investigated by immunostaining of TLC. The antigenic activities detected by immunofluorescence of cell smears were reflected in the antigenicities of high-MW glycoproteins. These were polydisperse and markedly susceptible to digestion with endo-.beta.-galactosidase. Only the I antigen was detected on minor glycolpids. Glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cells lines.This publication has 53 references indexed in Scilit:
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