The enzymatic activities in the alkali-Burnt rabbit cornea
- 1 March 1982
- journal article
- research article
- Published by Springer Nature in Albrecht von Graefes Archiv für Ophthalmologie
- Vol. 218 (3) , 145-148
- https://doi.org/10.1007/bf02215652
Abstract
Alkali-burnt corneas of the rabbits with 1 N NaOH were studied periodically for enzymatic activities by biochemical methods. There was significant increase of aldolase (ALD) activity both in corneal epithelium and stroma 1, 2, 3 and 4 weeks after alkali burns. Glucose-6-phosphate dehydrogenase (G6PD) activity was significantly decreased in epithelium and was absent in stroma. Thus the breakdown of glucose would be present preferably in the Embden-Meyerhoff pathway instead of the pentose phosphate shunt. Lactate dehydrogenase (LDH) activity of corneal epithelium and stroma was significantly decreased 1, 2, 3, and 4 weeks after alkali burns and the possible pathway of glycolysis might channel to citric acid cycle, in which malate dehydrogenase (MDH) could indicate the important role in this pathway.Keywords
This publication has 11 references indexed in Scilit:
- Corneal Glucose FluxArchives of Ophthalmology (1950), 1971
- Steady State Levels of Glucose in the Different Layers of the Cornea, Aqueous Humor, Blood and Tears in vivoOphthalmologica, 1967
- Beitrag zur biochemischen Wirkung des IUDR auf die HornhautAlbrecht von Graefes Archiv für Ophthalmologie, 1965
- The oxidation of C14-labeled glucose and lactate by the rabbit corneaArchives of Biochemistry and Biophysics, 1959
- Pathways of Glucose Metabolism in Corneal EpitheliumScience, 1955
- Glycolysis in the cornea of the rabbitThe Journal of Physiology, 1954
- The direct oxidative carbohydrate cycle of bovine corneal epitheliumArchives of Biochemistry and Biophysics, 1954
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- GLYCOLYTIC ACTIVITY OF THE CORNEAArchives of Ophthalmology (1950), 1951
- RESPIRATION OF THE CORNEAArchives of Ophthalmology (1950), 1950