Use of Antitryptic Agents in Tissue Culture. I. Crude Soybean Trypsin-Inhibitor.

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Abstract
Crude soybean trypsin-inhibitor prevents digestion of plasma and fibrinogen-thrombin clots by tissues growing in vitro. The presence of antitrypsin retards the normal coagulation of plasma but this inhibition can be overcome by the addition of thrombin. The growth of strain cultures of fibroblast-like cells is somewhat depressed by high concns. of antitrypsin, but the inhibitory effect can be eliminated by repeated cultivation in antitrypsin. Fresh tissue explants are not as sensitive as strain cultures to this inhibitory action.

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