Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence
- 1 November 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 5 (6) , 605-608
- https://doi.org/10.1093/mutage/5.6.605
Abstract
The Chinese hamster hypoxanthine—guanine phosphoribosyl-transferase (HPRT)-deficient cell line TG15 produces apparently normal HPRT mRNA by northern analysis and was therefore presumed to contain a point mutation within the coding region. Sequencing cDNA from the TG15 cell line revealed an A to G transition which results in the substitution of the amino acid glycine for aspartic acid at position 135. TG15 cells revert to wild-type HPRT activity upon exposure to monofunctional alkylating agents. A rapid test to assay the site of the TG15 point mutation has been developed, utilizing the polymerase chain reaction and allele-specific oligonucleotide screening. In all revertants studied, the original point mutation had been corrected to the wild-type sequence. The TG15 point mutation lies within a proposed catalytic domain of the HPRT protein in common with other phosphoribosyltransferases.This publication has 1 reference indexed in Scilit:
- Amplification versus mutation as a mechanism for reversion of anHGPRT mutationSomatic Cell and Molecular Genetics, 1984