DNA Polymerases from Intracisternal A‐Type Particles of the Mouse Plasmacytoma MPC 11

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Abstract
Intracisternal A particles from mouse plasma cell tumors were isolated from microsomal vesicles by detergent treatment and separated in linear sucrose gradients. Four peaks of DNA polymerase activities banding at densities of 1.30, 1.24, 1.20-1.22 and 1.14 g/cm3 were observed assaying on poly(rA).cntdot.oligo(dT). Solubilized DNA polymerases of the 1.30 g/cm3 fraction were eluted from phosphocellulose at salt concentrations around 0.30, 0.5 and 0.65 M KCl. They could be distinguished by their different responses towards poly(rA).cntdot.oligo(dT), poly(rC).cntdot.oligo(dG) and poly-(dA-dT). Serological experiments on the 3 DNA polymerases with antisera directed against Rauscher leukemia virus reverse transcriptase showed that only enzymes responding to poly(rA).cntdot.oligo(dT) and poly(rC).cntdot.oligo(dG) could be inhibited.