An Automated Method for Serum Calcium Utilizing Ethylene Diamine Tetraacetic Acid

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Abstract
An improved method of estimating serum calcium by the AutoAnalyzer using the reagent glyoxal-bis-(2-hydroxyanil) is described. Sensitivity and accuracy in the physiological range are increased by the use of ethylene diamine tetraacetic acid. Results correlate well with an established manual procedure and there is no interference from magnesium or phosphate.