Abstract
Superovulated does were inseminated with a minimal effective number of sperm suspended in Fructose Ringer, or seminal plasma of human, bull or rabbit; or in Fructose Ringer suspension of egg yolk, or deep frozen sperm. It was found that the seminal plasma of human or rabbit had a beneficial effect while that of bull had an ill effect on the fertilizing capacity of rabbit sperm as compared with Fructose Ringer. Fructose Ringer containing dead sperm of human, rabbit or bull was found as good or better media for the preservation of sperm as egg yolk; which indicates the presence of essential substances in the sperm cells for the maintenance of their fertilizing capacity.
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