Spin-Labeled Oligonucleotides Site Specifically Labeled at the Internucleotide Linkage. Separation of Stereoisomeric Probes and EPR Spectroscopical Detection of Hybrid Formation in Solution
- 1 February 1992
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 11 (2-4) , 889-901
- https://doi.org/10.1080/07328319208021747
Abstract
Described herein is the synthesis, separation of stereoisomers, hybridization, and EPR behavior of spin-labeled oligonucleotides site specifically labeled at the internucleotide linkage with 4-amino-TEMPO. The results obtained indicate that the spin-labeling method will provide a DNA probe useful for detecting hybrid formation with the complementary segment in solution.Keywords
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