Protein Storing Vacuoles in Ray Ceils of Willow Wood (Salix Caprea L.)

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Abstract
Light- and electron-microscopical investigations revealed protein bodies of c. 0.5 to 2.5 µm in diameter in the ray cells of willow wood. They consist of electron-dense aggregatesofvarious structural organisation which are enclosed in small-sized vacuole-like compartrnents. In semi-thin sections these aggregates showed positive protein staining with Ponçeau Red and Coomassie Blue, and enzymatic digestibility with pepsin. Because these protein bodies are found during the dormant season but not during summer, they are believed to be specific sites of protein storage in the ray cells of the wood. This is in accordance with the biochemical protein determination which yielded 6.4 to 8.4 µg mg-1 dry weight in late fall but only 1.2 to 2.0 µg mg-1 dry weight during summer. Light- and electron-microscopical investigations revealed protein bodies of c. 0.5 to 2.5 µm in diameter in the ray cells of willow wood. They consist of electron-dense aggregatesofvarious structural organisation which are enclosed in small-sized vacuole-like compartrnents. In semi-thin sections these aggregates showed positive protein staining with Ponçeau Red and Coomassie Blue, and enzymatic digestibility with pepsin. Because these protein bodies are found during the dormant season but not during summer, they are believed to be specific sites of protein storage in the ray cells of the wood. This is in accordance with the biochemical protein determination which yielded 6.4 to 8.4 µg mg-1 dry weight in late fall but only 1.2 to 2.0 µg mg-1 dry weight during summer.

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