Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca
- 4 July 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 234 (1) , 100-106
- https://doi.org/10.1016/0014-5793(88)81312-7
Abstract
Methods for isolation and electrophoretic analysis of surface proteins from the mucoraceous fungus Absidia glauca are described. By analysing the set of cell wall associated proteins which can be extracted with 1 M LiCl, we could show marked differences between submersed and aerial mycelium. Furthermore, during differentiation of the aerial mycelium protein patterns change remarkably. We were able to demonstrate the existence of mating type specific proteins, the most prominent of which is a 15 kDa polypeptide expressed in submersed as well as in aerial hyphae of the (+) mating type.Keywords
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